Formatted Title
Biotransformation of 6:2/4:2 Fluorotelomer Alcohols by Dietzia aurantiaca J3
Background/Objectives
Per- and polyfluoroalkyl substances (PFAS) and perfluorocarboxylic acids (PFCAs) are recalcitrant synthetic organohalides known to negatively impact human health. Short-chain fluorotelomer alcohols are considered the precursor of various PFCAs in the environment. Their ongoing production and widespread detection motivate investigations of their biological transformation.
Approach/Activities
Dietzia aurantiaca Strain J3 was isolated from PFAS-contaminated landfill leachate using 6:2FTS as a sulfur source. Resting cell experiments were used to test if Strain J3 could transform 6:2 and 4:2 FTOH. Fluoride ion, 6:2/4:2 FTOH and transformed product concentration were analysed by HPLC-ion chromatography (IC), gas chromatograph (GC-FID) and LC-HRMS (Q-Exactive HF Mass Spectrometer). Proteomics and enzyme inhibition studies were used to implicate specific enzymes in the biotransformation.