Topic Area: 1h. Optimization of Classical Bioremediation Technologies

Formatted Title:

Optimization of In Situ Bioremediation of Chlorinated Solvents Using Various Substrates and Bioaugmentation

Background/Objectives:

The purpose of the in situ bioremediation (ISB) system was to promote reductive dechlorination of chlorinated ethenes in groundwater via biostimulation in combination with bioaugmentation in the source areas. There are elevated concentrations of various chlorinated volatile organic compounds (cVOCs): tetrachloroethene (PCE), trichloroethene (TCE), and cis-1,2-dichloroethene (cis-1,2-DCE) in site groundwater. A variety of ISB approaches have been applied to the site using different carbon substrates.

Approach/Activities:

Three different areas of interest were identified at the site and a series of recirculation wells were installed in each area to address the higher concentrations of cVOCs. Optimization of the ISB system included conversion of the existing extraction wells to additional injection wells to increase the injection footprint; installation of additional injection wells; transition from a quick release to a slow-release carbon substrate to provide a long-lasting carbon source; and bioaugmentation with a Dehalococcoides microbial consortium to encourage microbial degradation of cVOCS. A bromide tracer study was also performed to evaluate the radius of influence of the injected amendments. A monitoring program was developed to evaluate the effectiveness of the treatment system, which included field parameters, cVOCs, key microbial populations and genes, geochemistry and field parameters.

Elena Dadukova

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(Group 2, Poster Board #80) Optimization of In Situ Bioremediation of Chlorinated Solvents Using Various Substrates and Bioaugmentation